rabbit anti solute carrier family 7 member 11  (Proteintech)

Journal: Cancers

Article Title: BUB1 Promotes Gemcitabine Resistance in Pancreatic Cancer Cells by Inhibiting Ferroptosis.

doi: 10.3390/cancers16081540

Figure Lengend Snippet: Figure 2. Erastin downregulates the expression of BUB1 and GPX4 while upregulating the expression of SLC7A11 in PC cell lines. (A) mRNA expression levels of BUB1 in different PC cell lines and healthy pancreatic cells detected using PCR, compared with HPNE, *** p < 0.001; (B−D) IC50 values of different PC cell lines determined using CCK8 assay; (E,F) mRNA and protein expression levels of BUB1, GPX4, and SLC7A11 in PANC−1 cells detected using PCR and Western blot, compared with NC, ** p < 0.01; (G,H) mRNA and protein expression levels of BUB1, GPX4, and SLC7A11 in MIA Paca-2 cells detected using PCR and Western blot, compared with NC, ** p < 0.01, *** p < 0.001. The uncropped blots are shown in Supplementary Materials.

Article Snippet: The antibodies used in this study were BUB1 (Abcam, Cambridge, UK, ab195268), Glutathione Peroxidase 4 (GPX4) (Abcam, ab125066), Solute Carrier Family 7 Member 11 (SLC7A11) (Cell Signaling Technology, Danvers, MA, USA, #12691), β-actin (Cell Signaling Technology, #3700), NF2 (Cell Signaling Technology, #12888), MOB1 (Cell Signaling Technology, #3863), YAP (Cell Signaling Technology, #14074), and GAPDH (Boster, Pleasanton, CA, USA, A00227-1).

Techniques: Expressing, CCK-8 Assay, Western Blot